Researchers: Pei Wang, Meiting Chen, Yaying Hou, Jun Luan, Ruili Liu, Liuqing Chen, Min Hu, Qiuliyang Yu
Nicotinamide adenine dinucleotide (NAD+ ) level has been associated with various age-related diseases and its pharmacological modulation emerges as a potential approach for aging intervention. But human NAD+ landscape exhibits large heterogeneity. The lack of rapid, low-cost assays limits the establishment of whole-blood NAD+ baseline and the development of personalized therapies, especially for those with poor responses towards conventional NAD+ supplementations. Here, we developed an automated NAD+ analyzer for the rapid measurement of NAD+ with 5 μL of capillary blood using recombinant bioluminescent sensor protein and automated optical reader. The minimal invasiveness of the assay allowed a frequent and decentralized mapping of real-world NAD+ dynamics. We showed that aerobic sport and NMN supplementation increased whole-blood NAD+ and that male on average has higher NAD+ than female before the age of 50. We further revealed the long-term stability of human NAD+ baseline over 100 days and identified major real-world NAD+ -modulating behaviors.
References
- NAD+ in Brain Aging and Neurodegenerative Disorders.
- Current Uncertainties and Future Challenges Regarding NAD+ Boosting Strategies.
- Therapeutic Potential of NAD-Boosting Molecules: The In Vivo Evidence.
- Association of Human Whole Blood NAD+ Contents With Aging.
- Targeting NAD+: is it a common strategy to delay heart aging?
Topic: Cost of aging care